![]() Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. They also reduce the mitochondrial membrane potential in mammalian cells. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). The sequence used for immunization shows 100% homology to mouse and rat UCP2 and 92% homology with canine, porcine and bovine. SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. UCP2 has wide tissue distribution in tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). It is only 59% homologous with UCP1 found in brown adipose tissues. Human UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. Please refer to the Certificate of Analysis for the lot-specific concentration. ![]() The peptide has no significant homology with UCP1, UCP3 or UCP4 (Boss et al. Optimal working dilutions must be determined by end user.ġ3 aa peptide sequence near the 3rd and 4th transmembrane domain of human UCP2 (Fleury et al. It is recommended that you use 2-20 μg/mL on formaldehyde fixed tissue.ĮLISA: 1:10,000-1:100,000 using 50-100 ng UCP2 control peptide/well. Immunohistochemistry: Has not been tested. Western Blotting: 1-10 μg/mL using Chemiluminescence technique. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA as stabilizer and 0.05% sodium azide.ĭetect Uncoupling Protein 2 using this Anti-Uncoupling Protein 2 Antibody validated for use in ELISA & WB. 100% Performance Guaranteed Key Spec Table Species ReactivityĪffinity purified immunoglobulin.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |